2026-10-14T22:54:50Z https://www.scidb.cn/oai

10.11922/sciencedb.01436 2022-01-11T09:53:09Z

2022-01-11 The sequencing results of PCR products doi:10.11922/sciencedb.01436 en We chose 17 representative species for confirming the IR region expansion/contraction. The four junctions between LSC/IRs and SSC/IRs of the 17 species were confirmed with PCR-based product sequencing. Target DNA regions were amplified in 25 μl reactions containing 10 ng (1 μl) template DNA, dNTP mixture 2 μl, 10 × LA PCR Buffer 2.5 μl, 0.5 μl of each primer, and 18.5 μl ddH2O. PCR products were bidirectionally sequenced by GENEWIZ, Inc.  Musaceae; IR border; PCR product Ning Fu PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1186/s12864-022-08454-3 Science Data Bank