2026-10-13T02:28:44Z https://www.scidb.cn/oai

10.11922/sciencedb.o00019.00013 2023-12-21T10:21:58Z

2023-12-21 Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture (maca_RNA_rawdata) doi:10.11922/sciencedb.o00019.00013 en Neural tube (NT) defects arise from abnormal neurulation and result in the most common birth defects worldwide. Yet, mechanisms of primate neurulation remain largely unknown due to prohibitions on human embryo research and limitations of available model systems. Here, we establish a three-dimensional (3D) prolonged in vitro culture (pIVC) system supporting cynomolgus monkey embryo development from 7 to 25 days post fertilization. Through single-cell multi-omics analyses, we demonstrate pIVC embryos form three germ layers, including primordial germ cells, and establish proper DNA methylation and chromatin accessibility through advanced gastrulation stages. In addition, pIVC embryo immunofluorescence confirms neural crest formation, NT closure and neural progenitor regionalization. Finally, we demonstrate that the transcriptional profiles and morphogenetics of pIVC embryos resemble key features of similarly staged in vivo cynomolgus and human embryos. This work therefore describes a system to study non-human primate embryogenesis through advanced gastrulation and early neurulation. Early embryonic development; Neurulation; Neural tube; Non-human Primate; Cynomolgus monkey; Advanced gastrulation; Single-cell Multi-omics; Three germ layers Jinglei Zhai Yanhong Xu Haifeng Wan Rui Yan Jing Guo Robin Skory Long Yan Xulun Wu Fengyuan Sun Gang Chen Wentao Zhao Kunyuan Yu Wei Li Fan Guo Nicolas Plachta Hongmei Wang PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1016/j.cell.2023.04.019 Science Data Bank