2026-10-14T16:50:17Z https://www.scidb.cn/oai

10.11922/sciencedb.o00061.00006 2022-03-21T17:53:53Z

2022-03-21 The 16srRNA data of EcN C6 on gut microbes in the hyperuricemia rat model. doi:10.11922/sciencedb.o00061.00006 en Feces collected from hyperuricemia rats were frozen in -80 ℃ until use. DNA was extracted using the E.Z.N.A. Stool DNA Kit (OMEGA, USA) following the instructions. 16S rDNA (V4 region) was amplified by a two-step PCR enrichment using barcodes for multiplexing. The pooled DNA was cleaned utilizing Ampure XP beads (Beckman, USA). DNA libraries were constructed using NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and were sequenced by Illumina HiSeq X Ten platform. 16srRNA; EcN C6; gut microbes; hyperuricemia He Lina Tang Wei Huang Ling ZhouWei Zou Linxuan Yuan Lisha Huang Shaojia Men Dong Chen Shiyun Hu Yangbo PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank