2026-10-14T19:44:21Z https://www.scidb.cn/oai

10.57760/sciencedb.01035 2026-08-31T10:12:02Z

2026-08-31 Cinnamaldehyde inhibits porcine coronaviruses infection by targeting HIF-1α doi:10.57760/sciencedb.01035 en Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, dehydration, vomiting and high mortality in newborn piglets, resulting in considerable economic losses in the global swine industry. Despite the application of multiple control strategies, PEDV remains widely prevalent. Therefore, it is urgent to develop effective antiviral strategies. In this study, using porcine intestinal organoids as an ex vivo screening platform, we evaluated 36 plant extracts for anti-PEDV activity and identified cinnamaldehyde (CA) as the most potent candidate, with a selectivity index (SI) of 189.67. Mechanistic investigations revealed that CA binds directly to HIF-1α and suppresses its transcription, thereby abolishing glycolysis-mediated inhibition of interferon (IFN) responses to inhibit PEDV replication. This mechanism not only effectively restricts PEDV replication but also exerts broad-spectrum antiviral activity against transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV). Importantly, In vivo experiments showed that CA inhibits PEDV replication in wild‑type (WT) mice, an effect that was abrogated in Ifnαr⁻/⁻ mice. Furthermore, CA restrains PEDV replication in piglets by abolishing HIF‑1α‑mediated inhibition of IFN responses. This study first demonstrates that CA enhances IFN responses to inhibit porcine coronaviruses replication by lowering HIF‑1α-glycolysis pathway. It provides new candidate drugs for the prevention and control of PEDV and other porcine coronaviruses. CA; intestinal organoids; PEDV; HIF 1α; IFN responses. Yunhang Zhang Yunchong Zhang Wanzhe Yuan EMBARGO https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank