2026-10-10T23:36:31Z https://www.scidb.cn/oai

10.57760/sciencedb.010vx 2026-09-02T15:53:44Z

2026-09-02 Tetrandrine improves cognitive dysfunction induced by adolescent social isolation via regulating microglia polarization in mice doi:10.57760/sciencedb.010vx en This study aims to investigate the impact of tetrandrine on cognitive dysfunction caused by adolescent social isolation. Mice in the SI group were individually housed for 9 weeks, whereas those in the GH group were kept four per cage. Tetrandrine was administered to mice in the SI+Tet group at 30 mg/kg (i.p.) starting 7 weeks post-social isolation. The novel object recognition was used to examine cognitive function. The IBA-1 expression was assessed using immunohistochemistry staining. The iNOS and ARG1 expression were analyzed through qRT-PCR. Western blot was used to detect the expression of IL-18, TNF-a, PSD-95 and SYN-1. The SI group mice demonstrated a pronounced reduction in the discrimination ratio during the novel object recognition, microglial process length, mRNA expression of the M2 macrophage marker ARG1, and protein expression of PSD-95 and SYN-1 compared to GH mice. Conversely, the number and cell body size of IBA-1+ microglial cells, mRNA expression of M1 macrophage marker INOS, and protein expression of IL-18 and TNF-a were increased. However, after the administration of tetrandrine, all of the above phenomena were significantly improved. Tetrandrine mitigates adolescent social isolation induced cognitive dysfunction by modulating microglial polarization and suppressing inflammatory factor release, thereby safeguarding neural synapses. Tetrandrine; social isolation; cognitive dysfunction; microglia; inflammatory factors; synapsis Zhen Wang PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank