2026-10-12T16:45:41Z https://www.scidb.cn/oai

10.57760/sciencedb.010xx 2026-09-13T20:08:07Z

2026-09-13 Raw Western blot data of baicalein inhibiting MAPK/ERK-FOS pathway in intervertebral disc degeneration doi:10.57760/sciencedb.010xx en This dataset contains raw Western blot (WB) images supporting the research article "Baicalein ameliorates intervertebral disc degeneration by inhibiting the MAPK/ERK-FOS pathway". Data are organized by figure (Figure 3-5, 7) and include 13 target proteins: GAPDH (loading control), COL2A1, Bcl-2, Caspase-3, Bax, FOS, ERK1/2, p-ERK1/2, MEK1/2, p-MEK1/2, c-Raf, p-c-Raf (Ser338), and Ras.Experimental design: Figure 3: human degenerated vs. normal NP tissue (n=3). Figure 4: TNF-α-treated human NP cells (n=3). Figure 5: baicalein treatment and FOS overexpression in TNF-α-stimulated NP cells (Control, TNF-α, TNF-α+baicalein 50 µM, TNF-α+baicalein+oeFOS; n=3). Figure 7: rat caudal disc degeneration model (Sham, AFP, AFP+GelMA, AFP+Bai@GelMA; n=3).Methods: Protein extracts were separated by SDS-PAGE, transferred to PVDF membranes, and probed with primary antibodies from Abcam (GAPDH ab8245, COL2A1 ab307674, Bcl-2 ab182858, Caspase-3 ab184787, Bax ab32503, FOS ab208942, ERK1/2 ab184699, p-ERK1/2 ab201015, MEK1/2 ab178876, p-MEK1/2 ab194754, c-Raf ab137435, Ras ab52939) and Cell Signaling Technology (p-c-Raf Ser338 #9427). Molecular weights were estimated using PageRuler Prestained Protein Ladder (Thermo, 26617). Signals were detected by BeyoECL Plus (P0018S) on ChemiDoc Go (Bio-Rad).File organization: Files are named as Fig{X}_{group}_{protein}_{replicate}.Tif. README.md provides detailed molecular weight annotations and explanations for all non-specific bands.Data processing: Band intensities were quantified using ImageJ. Phosphorylated protein levels were normalized to total protein and GAPDH. No data are missing. All raw images are unmodified full membrane exposures. Western blot; Intervertebral disc degeneration; Baicalein; MAPK/ERK; FOS; Nucleus pulposus; Apoptosis liumingli Qingyu Zhang PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank