2026-10-13T19:50:10Z https://www.scidb.cn/oai

10.57760/sciencedb.011bf 2026-09-04T16:16:57Z

2026-09-04 Salt-Assisted High Hydrostatic Pressure Strategy to Achieve High-Efficiency Non-Destructive Bacterial In-activation for Liquid Collagen doi:10.57760/sciencedb.011bf en Collagen and its derivatives stand out as valuable biomaterials for applications in foods, cosmetics, healthcare produce, medical equipment and drug delivery carriers due to its high biocompatibility, biodegradability, and lack of toxicity and immunogenicity. However, the problem of microbial contamination in collagen is too important to be ignored, which limiting their further application in various fields. Traditional bacterial in-activation methods such as heating and irradiation can effectively kill microorganisms in solid collagen, yet struggle to eliminate microbes in liquid collagen while preserving its biological activity. How to completely eradicate microorganisms in liquid collagen without loss of bioactivity for collagen, namely non-destructive bacterial in-activation, has become a key scientific challenge in the field of biomaterials. To answer this challenge, this study attempts to develop a bacterial in-activation strategy based on high hydrostatic pressure (HHP) technology to achieve non-destructive bacterial in-activation of Escherichia coli (E. coli)-contaminated liquid collagen. Although HHP treatment alone cannot in-activate E. coli, its bacterial in-activation efficacy can be enhanced by appropriately adjusting the environmental parameters of liquid collagen. The obtained experimental results presented that E. coli, incubated in a 0.9-3.0% sodium chloride solution for 6 to 12 hours and followed by HHP treatment at 300 MPa, could be eliminated, which indicating the great potential of salt-assisted HHP for bacterial in-activation. Meanwhile, the triple-helical structure characterization of liquid collagen, which was characterized by circular dichroism (CD) spectrometer and differential scanning calorimeter (DSC), could be saved under above same HHP treatment conditions. Finally, the non-destructive bacterial in-activation effect from salt-assisted HHP could also be observed in E. coli-contaminated liquid collagen model. We hope that this work can provide a feasible strategy to achieve high-efficiency non-destructive bacterial in-activation for liquid collagen. Collagen; High hydrostatic pressure; Escherichia coli; Sodium chloride; Non-destructive Bacterial In-activation. Zhenhu Guo Yan Zhang Jinyan Zhang Yang Zhang Lingyun Zhao Guifeng Zhang PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank