2026-10-11T20:36:01Z https://www.scidb.cn/oai

10.57760/sciencedb.012rc 2026-09-17T11:24:15Z

2026-09-17 Multi-omics and preclinical dataset supporting context-dependent immunomodulation by Clostridium butyricum extracellular vesicles in DSS (Th1/Th17) and oxazolone (Th2) colitis models doi:10.57760/sciencedb.012rc en This project deposited raw, paired-end 16S rRNA amplicon sequencing data generated to investigate gut microbial alterations in murine colitis models receiving Clostridium butyricum MIYAIRI 588 or C. butyricum extracellular vesicles. The study comprised two independent murine cohorts. In the DSS-driven Th1/Th17-skewed cohort, specific-pathogen-free male C57BL/6 mice were assigned to control, DSS-induced colitis, or DSS plus C. butyricum extracellular vesicles groups, with four biological replicates per group. In the oxazolone-driven Th2-skewed cohort, specific-pathogen-free male BALB/c mice were assigned to control, OXA, OXA plus C. butyricum, and OXA plus C. butyricum extracellular vesicles groups, with ten biological replicates per group. Fecal samples were collected from each animal, and DNA was extracted using DNA extraction kit. The V3-V4 region of the bacterial 16S rRNA gene was amplified with primers 338F and 806R, followed by library preparation and sequencing on an Illumina MiSeq platform in 2x250 bp mode. This BioProject includes 12 samples from the DSS cohort and 40 samples from the OXA cohort, totaling 52 host-associated fecal samples. Clostridium butyricum; extracellular vesicles; 16S rRNA; gut microbiota; Th2 colitis; oxazolone; DSS; dendritic cells; autophagy; LC3B Shun Zhang Jinbo Wang PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank