2026-10-14T17:44:00Z https://www.scidb.cn/oai

10.57760/sciencedb.013su 2026-09-28T15:57:24Z

2026-09-28 A Single-Cell Transcriptomic Dataset of the Neonatal Mouse Cortex Following Tenascin-C Deletion doi:10.57760/sciencedb.013su en OverviewThis dataset contains single-cell RNA-sequencing data from the dorsal cerebral cortex of postnatal day 0 (P0) mice, comprising three wild-type control (Ctrl) and three Tnc conditional knockout (KO) samples. Each sequencing sample was generated by pooling dorsal cortical tissues from at least six littermate pups.The Tnc conditional knockout model was generated on a C57BL/6JCya background using the C57BL/6JCya-Tncem1flox/Cya conditional knockout strain. The engineered Tnc allele contains loxP sites flanking the targeted region, enabling Cre-mediated deletion. Tnc was conditionally deleted using Emx1-Cre, resulting in conditional deletion of Tnc in the dorsal cortex.This dataset enables investigation of cell-type-specific transcriptional changes associated with Tnc loss during early postnatal cortical development.Dataset ContentsThis dataset includes:Raw sequencing data for all samples in compressed FASTQ (.fastq.gz) format.MD5 checksum files corresponding to each FASTQ file for file integrity verification.File FormatEach biological replicate is stored in a separate folder. Folder names follow the format Group-Sample_raw. Group denotes the experimental group (Ctrl or KO), and Sample denotes the biological replicate number.FASTQ files are stored in the corresponding sample folder. File names follow the format Group-Sample_S1_L00X_RY_001.fastq.gz. L00X denotes the sequencing lane. R1 and R2 denote paired-end reads.The MD5 checksum file is stored in the same folder. It is named Group-Sample_md5.txt and contains checksums for the FASTQ files of the corresponding biological replicate. single-cell RNA-sequencing; mouse cortex; Tnc deletion Zirun Li Xinwei Tan Shuting Ling Wei Huang PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank