2026-10-12T10:14:47Z https://www.scidb.cn/oai

10.57760/sciencedb.01889 2024-02-29T20:19:58Z

2024-02-29 Simulation analysis and physiological and biochemical evaluation of Sophora flavescens Alt. aboveground against aphid based on network pharmacology doi:10.57760/sciencedb.01889 en The aboveground part of Sophora flavescens was dried, crushed, filtered through a 0.25-mm (60 mesh) sieve, mixed with 60% ethanol containing 0.02% ammonia (1:6), and stirred overnight at 60 °C. The extract was sonicated using 19-kHz ultrasonic waves, and filtered. The filtrate was collected and the solid portion was re-extracted as described above. Subsequently, the water in the filtrate was removed by vacuum rotary evaporation. The crude extract of the aboveground part of Sophora flavescens was obtained by centrifugation at 3,000 rpm for 10 minutes. Myzus persicae Sulzer (M. persicae) and Aphis craccivora Koch (Aphis craccivora) were reared at 25 °C and 70% humidity (Akca et al. 2015; Mseddi et al. 2022). The host plants Brassica campestris L. ssp. chinensis Makino (var. communis Tsen et Lee) (A), Datura stramonium Linn. (B) and Astragalus membranaceus (Fisch.) Bunge (Astragalus membranaceus) were reared at low density. The photoperiod was set at 12:12 (light:dark) (Colizzi et al. 2021; Nakazato et al. 2021). M. persicae grown on pakchoi and mandala were used as the experimental insects. Aphis craccivora grown on Astragalus membranaceus used as the experimental insects. Uniform aphids were selected for the experiment. The crude extract of the aboveground part of Sophora flavescens, matrine, oxymatrine, sophocarpine, and sophoridine (95% purity) (Chengdu Desite Biotechnology Co., Ltd.) were dissolved in acetone solution containing 20% (Colizzi et al. 2021; Nakazato et al. 2021). The compounds were tested at 100 μM, 1 mM, 10 mM, and 100 mM to determine the activity of G6PD, TS, and TSPO using a kit (Jiangsu enzyme industry Co., Ltd) (Ma et al. 2020). Each experimental compound or blank (5 μL per aphid) was locally applied to early third instar aphids. Sixty aphids were treated per experiment, and each experiment was repeated 3 times. Aphids in the control group were only exposed to the solvent (20% acetone). G6PD activity was examined 12 and 24 h after treatment; TS activity was tested 0.5, 24, and 36 h after treatment, and TSPO activity was tested at 0.5, 12, and 24 h. The collected samples were frozen in liquid nitrogen for 10 minutes and stored in a freezer at -80 °C. The chemical composition-target interaction network between aphid disease and the aboveground part of Sophora flavescens was constructed using Cytoscape 3.7.2 software to explore the potential pharmacological mechanism of the identified compounds. In the network map, a node represents a drug, diseases, active ingredient, or target. Edges represent the relationship between active aphids, the aboveground parts of Sophora flavescens, and the molecular targets. aphid; insecticide; pesticide guo chun yan Minhui Li PUBLIC https://creativecommons.org/licenses/by-sa/4.0/ dataset Science Data Bank