2026-10-14T01:12:00Z https://www.scidb.cn/oai

10.57760/sciencedb.04557 2022-11-28T02:18:47Z

2022-11-28 Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli doi:10.57760/sciencedb.04557 en 2’-Fucosyllactose (2’-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2’-FL production. The highest 2’-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2’-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2’-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2’-FL titer reached a maximum value of 7.14 g/L. 2’-Fucosyllactose; Escherichia coli; acetate; culturing medium; induction conditions; glucose Yingxue Liao Zhijian Ni Jinyong Wu Zhongkui Li Yuanfei Ge Xiangsong Chen Jianming Yao PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/https://doi.org/10.1080/13102818.2021.1885996 Science Data Bank