2026-10-14T12:45:57Z https://www.scidb.cn/oai

10.57760/sciencedb.05611 2022-10-31T13:46:40Z

2022-10-31 Metagenome-assembled genomes infer potential microbial metabolism in alkaline sulphidic tailings doi:10.57760/sciencedb.05611 en In this study, we detected a considerable amount of viable bacterial and archaeal cells using fluorescent in situ hybridization in alkaline sulphidic tailings from Mt Isa, Queensland. By taking advantage of high-throughput sequencing and up-to-date metagenomic binning technology, we reconstructed the microbial community structure and potential coupled iron and nitrogen metabolism pathways in the tailings. Assembly of 10 metagenome-assembled genomes (MAGs), with 5 nearly complete, was achieved. From this, detailed insights into the community metabolic capabilities was derived. Dominant microbial species were seen to possess powerful resistance systems for osmotic, metal and oxidative stresses. Additionally, these community members had metabolic capabilities for sulphide oxidation, for causing increased salinity and metal release, and for leading to N depletion. tailings; Metagenome; microbial metabolism Wenjun Li Xiaofang Li PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1186/s40793-021-00380-3 Science Data Bank