2026-10-13T08:04:12Z https://www.scidb.cn/oai

10.57760/sciencedb.07431 2023-02-15T15:52:40Z

2023-02-15 Efficient Biosynthesis of Phycobiliprotein in Escherichia coli doi:10.57760/sciencedb.07431 en  In this work, a pathway for SLA-PEB (a fusion protein of streptavidin and allophycocyanin that covalently binds phycoerythrobilin (PEB)) biosynthesis in E. coli was constructed using a single-expression plasmid strategy. Compared with a previous E. coli strain transformed with dual plasmids, the E. coli strain transformed with a single plasmid showed increased plasmid stability and produced SLA-PEB with a higher chromophorylation ratio. To achieve full chromophorylation of SLA-PEB, directed evolution was employed to improve the catalytic performance of lyase CpcS. In addition, the catalytic abilities of heme oxygenases from different cyanobacteria were investigated based on biliverdin IXα and PEB accumulation. Upregulation of the heme biosynthetic pathway genes was also carried out to increase heme availability and PEB biosynthesis in E. coli. Fed-batch fermentation was conducted for the strain V5ALD, which produced recombinant SLA-PEB with a chromophorylation ratio of 96.7%. 藻胆蛋白; 藻胆色素; 质粒稳定性; 大肠杆菌 Huaxin Chen Peng Jiang PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset http://www.doi.org/https://doi.org/10.1186/s12934-019-1100-6 Science Data Bank