2026-10-10T19:09:49Z https://www.scidb.cn/oai

10.57760/sciencedb.07522 2023-02-23T17:02:11Z

2023-02-23 Clinical diagnostic analysis of 504 suspected tuberculosis cases by CRISPR/Cas technology doi:10.57760/sciencedb.07522 en MTB H37Ra could be detected as low as 3.13 CFU/mL by the CRISPR-Cas12a system targeting IS6110. With BACTCE960 culture (120 positives and 384 negatives) as the gold standard, the sensitivity of the TB-CRISPR technique was 0.883 (0.809-0.932) and the specificity was 0.940 (0.910-0.961). According to the receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) reached 0.944 (0.914-0.975) within 95% CI. The positive likelihood ratio (PLR) was 14.747 (9.870-22.035) and the negative likelihood ratio (NLR) was 0.124 (0.076-0.203). The positive predictive value (PPV) was 0.822 (0.742-0.881) and the negative predictive value (NPV) was 0.963 (0.937-0.979). CRISPR/Cas; tuberculosis; BACTEC960 Xiaoyu Zhang XiaoyingHe Yubo Zhang Lei Chen Zhaobao Pan Yueying Huang Heng Li PUBLIC https://api.github.com/licenses/unlicense dataset http://www.doi.org/10.21203/rs.3.rs-2588529/v1 Science Data Bank