2026-10-14T06:02:34Z https://www.scidb.cn/oai

10.57760/sciencedb.07856 2023-03-30T09:08:08Z

2023-03-30 donkey astrovirus DAstV-1 doi:10.57760/sciencedb.07856 en we collected intestinal samples from six deceased donkey foals, stored them at -80 ℃, and subsequently transported them to our laboratory for pathogen detection with virome. The samples were homogenized with steel beads and total RNA was extracted using an Omega viral DNA/RNA kit (Omega, USA). Next, a mixture of six nucleic acid samples was tested using nextgeneration sequencing. A sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA). The library was sequenced on an Illumina Novaseq 6000 (PE150) sequencing platform (San Diego, CA, USA). Novel astrovirus; Mamastrovirus; Donkey astrovirus Jing Li PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank