2026-10-12T04:30:47Z https://www.scidb.cn/oai

10.57760/sciencedb.08789 2024-06-18T15:32:24Z

2024-06-18 Mass spectrometry analysis of Flag-H9N2-NS2 interacting candidates doi:10.57760/sciencedb.08789 en To identify SUMOylated factors that interact with NS2 via SIM-SUMO interaction, FLAG-tagged H9N2-NS2 was co-transfected into HEK293T cells together with plasmids encoding His-SUMO1 and Myc-Ubc9. After transfection for 24 hours, cells were infected with H9N2 AIV for a further 24 hours and Flag-NS2 was purified using anti-Flag beads. Purified protein complexes were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Avian influenza virus H9N2 ; NS2; LC-MS/MS Xiaojun Wang Liuke Sun Xing Guo Mengmeng Yu Xue-Feng Wang Huiling Ren PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset http://www.doi.org/10.18256/2237-7956.2023.v13i1.4548 Science Data Bank