2026-10-14T21:05:22Z https://www.scidb.cn/oai

10.57760/sciencedb.09416 2024-01-22T13:57:17Z

2024-01-22 Genetic data from 4 leafless vanilla populations doi:10.57760/sciencedb.09416 en We collected 25 leafless vanilla samples from four sites {Ambatofaly (ABF), Ampitolova (APL), Vohitsara (VHT) and Ampotaka (APTK)}. We removed 15 cm of stems or young buds and preserved them in silica gel, obtaining a total of 100 individuals for the four populations. DNA extraction was performed using Qiagen's DNEASY® plant kit for all 100 specimens. Seven microsatellite markers developed by Gigant et al (2011) were used to amplify and genotype each individual. Polymerase chain reaction (PCR) with fluorescently-labeled primers was performed in a total volume of 25 μl using a Promega kit (Promega, 2007). The mixture contained 12.5 μl of GoTaq Colourless Master Mix 2X, 2 μl of DNA solution (5 < DNA < 250 ng), 0.4 μM of each primer and 8.5 μl of nuclease-free water. Capillary electrophoresis with an ABI 3130XL automated genetic analyzer (Applied Biosystems) was used to genotype PCR fragments. GENEIOUS PRIME 2019.1 was used for allele size coding. Leafless vanilla; Genetics; Orchid Botomanga Aro Vonjy Ramarosandratana PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank