2026-10-13T08:34:24Z https://www.scidb.cn/oai

10.57760/sciencedb.11864 2024-09-27T10:21:27Z

2024-09-27 Assessing the effect of foodborne endocrine disruptors exposure on methionine synthase activity in neuro-2a neuroblastoma cell lines. doi:10.57760/sciencedb.11864 en The potential impact of EDCs on MS, the activity of this enzyme in neuroblastoma (Neuro2A) cells with no exposure to EDC’s was measured obtaining a concentration of 2.3 ± 0.06 nmol/(min*mg of protein). This value was significantly higher when compared to Neuro2A cells exposed to endocrine disruptors.The MS specific activities observed on Neuro2A line cell exposed to DEHP, MBP and OP phthalates were 1.2 ± 0.06 nmol/(min* mg of protein), 0.79 ± 0.03 nmol/(min* mg of protein) and 1.3 ± 0.11 nmol/(min* mg of protein) respectively.On the other hand, BPA is a compound with two phenolic rings (benzene with an OH group) linked centrally to a propane moiety symmetrically. The observed MS activity in presence of this EDC was 1.51 ± 0.03 nmol/(min* mg of protein) as a result of 24 h exposure, which is significantly lower to control conditions while its effects were less marked than those of MBP. Endocrine Disrupting Compounds (EDCs); phthalates; bisphenol A (BPA); methionine synthase activity; in vitro model; biosensor Roeb García Gabriela García Cerón Alba Solano González Iván Bello Cortés Andrea Bonilla Brunner Daniela Araiza Olivera Manuel Gutiérrez Aguilar Yazmín Ramiro Cortés Marcos Rosetti PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank