2026-10-13T11:58:40Z https://www.scidb.cn/oai

10.57760/sciencedb.12290 2024-08-23T11:36:40Z

2024-08-23 The sequence information of BLR, C73, and HM26 doi:10.57760/sciencedb.12290 en Unveiling the molecular mechanisms underlying rotavirus replication and pathogenesis had been hampered by the lack of a reverse genetics (RG) system in the past. Since 2017, multiple plasmid-based RG systems for simian, human, and murine-Like rotaviruses have been established. However, none of the described methods have supported the recovery of bovine rotaviruses (BRVs). Here, an optimized plasmid-based RG system for BRV culture-adapted strain (BRV G10P [15] BLR) and clinical isolates (BRV G6P[1] C73, G10P[11] HM26) was established based on a BHK-T7 cell clone stably expressing T7 polymerase. Bovine rotavirus (BRVs); optimized reverse genetics system; reporter virus Xin Yin Jitao Chang Songkang Qin Kuanhao Li Benjin Liu Cun Cao Debin Yu Zhigang Jiang Songkang Qin Jun Wang Yuxin Han Fang Wang Yinglin Qi Chao Sun PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1016/j.virs.2024.09.010 Science Data Bank