2026-10-12T19:55:16Z https://www.scidb.cn/oai

10.57760/sciencedb.12511 2024-08-30T16:01:20Z

2024-08-30 Haplotype-resolved nonaploid genome provides insights into in vitro flowering in bamboo doi:10.57760/sciencedb.12511 en This dataset includes raw data, assembly, and annotation for the genome of Bambusa odashimae. The raw data consists of Next Generation Sequencing (NGS) data (NGS_*.fa.gz), Hi-C data (HIC*_raw_*.fa.gz), and Oxford Nanopore Technology (ONT) data (ONT*.pass.fastq.gz). The results of the assembly and annotation are provided in the following files: phased.bod.cds, phased.bod.gff, phased.bod.pep, and phased.genome.fasta. Below is a detailed description of each data source. Raw Data: Fresh leaves of B. odashimae were collected, and DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method. For NGS sequencing, PCR-free libraries were constructed and sequenced on the MGISEQ2000 platform. For ONT sequencing, high-quality DNA was extracted with the QIAGEN Genomic kit, followed by library construction with the LSK109 kit and sequencing on the ONT platform. To position hybrid scaffolds onto the chromosome, Hi-C samples were prepared, a Hi-C library was constructed, and data were obtained using the Illumina NovaSeq platform. Genome Assembly and Annotation Data: The methods for genome assembly and annotation are detailed in the article "Haplotype-resolved nonaploid genome provides insights into in vitro flowering in bamboos," which has been accepted by Horticulture Research.  Bambusa odashimae; genome; nonaploid Yu-Jiao Wang Cen Guo Lei Zhao Ling Mao Xiang-Zhou Hu Ke-Cheng Qian Peng-Fei Ma Zhen-Hua Guo De-Zhu Li PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank