2026-10-10T18:26:13Z https://www.scidb.cn/oai

10.57760/sciencedb.12594 2023-10-24T15:30:58Z

2023-10-24 RNA-seq data from tree shrew spermatogonia stem cells with different treatments doi:10.57760/sciencedb.12594 en Total RNA was extracted from undifferentiated tree shrew spermatogonia using TRNzol. Two independent biological samples were prepared. RNA-seq was performed on the Illumina NovaSeq 6000 system. Clean sequence reads were mapped to the tree shrew reference genome (KIZ v3) using TopHat2 software and coding gene expression was calculated using Cufflinks. Cuffdiff software was used to determine differentially expressed genes (DEGs). Gene Ontology (GO) enrichment analysis was performed using online tools (http://geneontology.org/). Heatmaps were draw using the “gplot” package in R.  tree shrew; spermatogonial stem cell; in vitro culture Cong li Yong-Gang Yao Ping Zheng Rui Bi Yu-Hua Ma Lin Wang PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank