2026-10-14T06:55:20Z https://www.scidb.cn/oai

10.57760/sciencedb.12899 2024-09-18T19:56:29Z

2024-09-18 Blocking virus infection with BacMam virus delivery bovine interferon-α gene（raw data） doi:10.57760/sciencedb.12899 en Interferon-αs (IFN-αs) are crucial cytokines for inducing protective antiviral responses. The baculovirus-mediated gene transduction of mammalian cells (BacMam) is an efficient delivery tool for recombinant protein expression in mammalian cells. This study focuses on the delivery of bovine IFN-α (BoIFNα) using the BacMam system. A recombinant pACEMam1-BoIFNα bacmid was constructed, and a recombinant BacMam-BoIFNα virus was obtained. After transducing HEK293T cells with this virus, BoIFNα protein was successfully secreted into the cell supernatant, displaying antiviral activities against VSV, BPIV3, BEV, and BVDV in bovine-derived cells. Additionally, the BacMam-BoIFNα virus inhibited the replication of these viruses in MDBK cells, induced the transcription of ISGs, and the expression of Mx1 in both MDBK and BT cells. These induction effects were significantly reduced following treatment with a JAK1 inhibitor, suggesting that the BacMam-BoIFNα virus exerts antiviral activity in MDBK cells through JAK-STAT signaling pathway. Furthermore, the study found that the BacMam-BoIFNα virus significantly inhibited the replication of BPIV3 in the lung and trachea of mice. Overall, this study demonstrates that the BacMam-BoIFNα virus have antivirus activities both in vitro and in vivo, signaling through JAK-STAT pathway, and provides an efficient interferon gene delivery tool for combating bovine viral infections. Bovine interferon-α; BacMam system; Antiviral activity; JAK-STAT signaling pathway; Inhibition of virus replication. Zhenying Fan Yajuan Hou Yue Liu Jingjing Zhao Yixiao Wang Chun Fu Shuangfeng Wang Junwei Wang Yongli Guo Mingchun Gao PUBLIC https://creativecommons.org/licenses/by-nc-sa/4.0/ dataset http://www.doi.org/10.1080/21505594.2024.2435372 Science Data Bank