2026-10-10T18:47:23Z https://www.scidb.cn/oai

10.57760/sciencedb.17013 2024-03-15T13:03:28Z

2024-03-15 SAFB restricts contact domain boundaries associated with L1 chimeric transcription doi:10.57760/sciencedb.17013 en Long interspersed element-1 (LINE-1 or L1) comprises 17% of the human genome,continuously generates genetic variations, and cause disease in certain cases. However, the regulation and function of L1 remain poorly understood. Here, we uncover that L1 can enrich RNA polymerase IIs (Pol IIs), express L1 chimeric transcripts andcreate contact domain boundaries in human cells. This impact of L1 is restricted by anuclear matrix protein Scaffold Attachment Factor B (SAFB) that recognizes transcriptionally active L1s by binding L1 transcripts to inhibit Pol II enrichment. Acute inhibition of Pol II transcription abolishes the domain boundaries associated with L1 chimeric transcripts, indicating a transcription-dependent mechanism. Deleting L1impairs domain boundary formation, and L1 insertions during evolution have introduced species-specific domain boundaries. Our data show that L1 can create Pol II enriched regions that alter genome organization, and that SAFB regulates L1 and Pol II activity to preserve gene regulation. LINE-1; Genome architecture; Contact domain; SAFB; Pol II; L1 chimeric RNA; RNA-binding protein; Retrotransposon; Evolution Yaqiang Hong PUBLIC https://api.github.com/licenses/unlicense dataset Science Data Bank