2026-10-14T16:48:03Z https://www.scidb.cn/oai

10.57760/sciencedb.22345 2025-03-21T11:00:27Z

2025-03-21 Original data (CT values) of RT-qPCR. doi:10.57760/sciencedb.22345 en The T. urticae mites, collected as described in section 2.6, were used for RNA extraction, following the protocol outlined in section 2.3. Total RNA was reverse transcribed into complementary DNA (cDNA) using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China). Based on the gene sequences obtained from cloning, specific primers for RT-qPCR were designed using the Primer3 Input online tool (refer to Table S1). For normalization of gene expression levels, the α-tubulin gene (GenBank Accession: JN881327.1) was selected as the internal reference gene. RT-qPCR was conducted on an ABI QuantStudio 5 Real-Time PCR System with Hieff UNICON qPCR SYBR Green Master Mix (Yeasen, Shanghai, China). Three technical replicates were performed for each biological replicate. Gene expression levels were quantified using the 2-ΔΔCt method  Tetranychus urticae; peroxidase; short-term heat stress; bioinformatics analysis; gene expression pattern Yao-Nian Chen Su-Qin Shang PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank