2026-10-14T20:41:11Z https://www.scidb.cn/oai

10.57760/sciencedb.22769 2025-03-27T10:26:57Z

2025-03-27 VGLL1 cooperates with TEAD4 to control human trophectoderm lineage specification doi:10.57760/sciencedb.22769 en Placenta abnormality is one of the key reasons for early pregnancy loss but the regulatory mechanisms underlying placenta formation are largely unknown. Here, we applied a human naïve pluripotent stem cell (PSC) derived TE model to explore the driven regulatory machinery. We demonstrate that VGLL1 (vestigial like family member 1) is crucial for TE self-renewal and TE-specific gene expression, thus suppressing VGLL1 leads to severely impaired cell proliferation and skewed TE induction. VGLL1 exerts its function through interacting with TEAD4 (TEA domain transcription factor 4) and colocalize at target gene promoters and enhancers. Further investigation uncovers the enrichment of H3K27ac active histone marks at genomic regions occupied by VGLL1-TEAD4 complex, indicates a close association between them. Overall, our data reveals the critical function model of VGLL1-TEAD4 in human TE derivation, highlighting a potential interspecies difference between human and mouse TE induction. Human trophectoderm-like cells; Human trophoblast stem cells; RNA-seq; ATAC-seq; CUT&Tag Yueli Yang Wenqi Jia Zhiwei Luo Yunpan Li Hao Liu Lixin Fu Jinxiu Li YuJiang Junjian Lai Haiwei Li Babangida Jabir Saeed Yi Zou Yuan Lv LiangWu Ting Zhou Yongli Shan Chuanyu Liu Yiwei Lai Longqi Liu Andrew P. Hutchins Miguel A. Esteban* Md. Abdul Mazid* Wenjuan Li* PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1038/s41467-025-57929-w Science Data Bank