2026-10-14T23:08:38Z https://www.scidb.cn/oai

10.57760/sciencedb.26264 2025-06-10T11:06:26Z

2025-06-10 A dataset of methylation profiles in EPC2 cells treated with 3% polycyclic aromatic hydrocarbons (PAHs) and wildtype EPC2 cells doi:10.57760/sciencedb.26264 en Library construction protocol: After the sample passes quality control, DNA samples are first subjected to digestion using the methylation-insensitive restriction enzyme MspI. The resulting DNA fragments are then subjected to end repair, A-tail addition, and ligation with sequencing adapters that are modified at all cytosines to ensure they are methylated. Gel electrophoresis is used to select DNA fragments with insert sizes ranging from 150 to 300 base pairs. The fragments are then treated with bisulfite (using the EZ DNA Methylation Gold Kit, Zymo Research). During this treatment, unmethylated cytosines are converted to uracil (which becomes thymine after PCR amplification), while methylated cytosines remain unchanged. Finally, PCR amplification is performed to generate the final DNA library.Devices: Illumina HiSeq PE150Data processing: Sequencing adaptors and low-quality reads were trimmed from the raw sequencing data to obtain clean data for subsequent analysis. The trimming was performed using Trimmomatic software.Data files format and content: The datasets provide methylation information for cytosine sites across the genome, including chromosome location, strand orientation, methylation context (CG, CHG, CHH), the number of methylated and unmethylated reads, and the calculated methylation ratio (β value).Organism: Homo sapiensGenome assembly: hg19 (NCBI-Assembly) Reduced Representation Bisulfite Sequencing; methylation; EPC2 jiaying zhang PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset Science Data Bank