2026-10-12T13:20:50Z https://www.scidb.cn/oai

10.57760/sciencedb.27202 2025-07-04T16:34:27Z

2025-07-04 Segmented filamentous bacteria confer protection against norovirus infection through enhanced viral clearance, immune modulation, and intestinal barrier function doi:10.57760/sciencedb.27202 en we demonstrate that SFB profoundly accelerates viral clearance. SFB-colonized mice exhibited significantly reduced fecal viral loads by day 5 post-infection (dpi) compared to GF and control bacteria mice (p < 0.001), reaching near-baseline levels by day 10. This enhanced viral control correlated with markedly attenuated morbidity; SFB mice experienced minimal weight loss and rapid recovery, while GF and control mice suffered severe weight loss and delayed recovery (p < 0.001). At peak infection (3 dpi), SFB colonization significantly suppressed viral replication in feces and tissues and attenuated intestinal pathology. SFB also preserved barrier integrity, evidenced by reduced serum FITC-dextran. Mechanistically, SFB induced a distinctive antiviral cytokine profile, characterized by elevated IFN-λ and IL-22, while tempering IFN-γ increases. Protection was dose-dependent, with pre-infection SFB abundance strongly inversely correlating with day 3 viral load and pathology. Integrated multi-parameter and principal component analyses revealed a unique SFB-driven protective state, featuring accelerated clearance, reduced pathology/inflammation, barrier preservation, and elevated protective cytokines, establishing a pre-primed mucosal environment. Segmented filamentous bacteria (SFB); Norovirus; Protective Mechanism; Immunomodulation; Intestinal Barrier Integrity NIGNBO LIAO PUBLIC https://opendatacommons.org/licenses/by/1-0/ dataset Science Data Bank