2026-10-12T03:08:03Z https://www.scidb.cn/oai

10.57760/sciencedb.28794 2025-09-02T11:12:02Z

2025-09-02 Brucella proline racemase protein A targets Tpl2 to promote IL-10 secretion for establishment of chronic infection doi:10.57760/sciencedb.28794 en This dataset includes the IL-10 concentration in macrophages after stimulation with rRomA, rOMP25, rwadC, and rPrpA proteins in vitro and in vivo. It also includes the IL-10 concentration in macrophages aftertransfection with varying doses of the pcDNA3.1-prpA plasmid. All IL-10 concentrations were determined using ELISA kits. Additionally, the dataset contains data on the number of cells induced by PrpA, as well as the proliferation rate of macrophages (evaluated using CCK-8 kits). Furthermore, the dataset includes data on the intracellular survival of M5-90, the M5-90 prpA mutant, and M5-90 prpA-c in murine macrophages. It also comprises proteomics data of murine macrophages infected with M5-90 and the M5-90 prpA mutant, along with results from molecular dynamics simulations of the PrpA and Tpl2 proteins. Finally, the dataset includes the following: 1) Spleen colony-forming units (CFUs) of mice inoculated with M5-90 and the M5-90 prpA mutant at 30 days post-inoculation; 2) Serum levels of Brucella-specific IgG antibody, IFN-γ, TNF-α, and IL-10 (evaluated using ELISA kits) at 30 and 45 days post-inoculation; 3) Flow cytometry analysis data of splenic lymphocytes from mice immunized with M5-90 and the M5-90 prpA mutant at 30 and 45 days post-immunization; 4) CFU counts in the spleen from challenge experiments. Brucella melitensis M5-90; proline racemase; tumor progression locus 2; IL-10; vaccine Huan Zhang Yuanzhi Wang Yueli Wang Huilin Hou Xiaoyu Deng Zhengyu Xu Xujin Xia Mingguo Xu Zhen Wang Changsuo Zhang Kait Zhumanov Jinliang Sheng Zhongchen Ma Jihai Yi Chuangfu Chen PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank