2026-10-15T02:02:41Z https://www.scidb.cn/oai

10.57760/sciencedb.28818 2025-08-07T11:32:19Z

2025-08-07 Raw data for the article "Glucose restriction induces degeneration of neurons with mitochondrial DNA depletion by altering ER-mitochondria calcium transfer" doi:10.57760/sciencedb.28818 en Total RNA was extracted with TRIzol. Libraries were prepared using an Illumina TruSeq RNA Sample Prep kit (Illumina, 20020594) following the manufacturer’s instruction. The RNA-seq experiment was performed at Annoroad Gene Technology Co. Ltd. (Beijing, China). The raw reads of RNA-seq were trimmed from 3′ to 5′ ends for each read, and the reads shorter than 50 bp were discarded. The reads that passed the quality control were mapped to mouse genome (mm10) with STAR (version 2.7). Stringtie (version 2.1.4) were then used to estimate transcript abundance in fragments per kilobase per million (FPKM). Genes were annotated as differentially expressed using DESeq2 (version 1.44). The GO analysis and KEGG pathway analysis were performed using R packages. mitochondria; Neurodegeneration; Mitochondrial DNA Lingyan Zhou Feixiang Bao Xingguo Liu PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1038/s41380-025-03069-y Science Data Bank