2026-10-14T08:33:03Z https://www.scidb.cn/oai

10.57760/sciencedb.29579 2025-10-13T17:41:14Z

2025-10-13 Chromosome-level genome assembly of the Pallas's cat (Otocolobus manul) doi:10.57760/sciencedb.29579 en This study presents a chromosome-level genome assembly of the Pallas's cat (Otocolobus manul) from a individual in Qinghai, integrating PacBio HiFi long-read sequencing, Illumina short-read sequencing, and Hi-C chromatin interaction mapping. Muscle tissue samples (Sample ID: FSD0220013820–1A) were flash-frozen in liquid nitrogen, with DNA extraction and quality assessment conducted by Novogene Bioinformatics Technology Co., Ltd. A total of 134 Gb PacBio HiFi reads were generated on the Sequel II platform, while Illumina libraries (350 bp insert size) and Hi-C libraries (using Mbol digestion) were sequenced with PE150 reads. The genome was assembled using hifasm, polished with Pilon, Racon, Arrow, and NextPolish, and scaffolded into 19 pseudo-chromosomes via HiCUP and ALLHIC, achieving 98.02% anchoring rate. The assembly exhibits high completeness (97.6% BUSCO score) and accuracy (Mercury QV = 50.46). Genome annotation identified 41.09% transposable elements and 22,699 protein-coding genes, with functional annotations covering 90.37% of genes. The dataset includes genome sequences, annotation files, and raw sequencing data. Otocolobus manul; Chromosome-level Assembly; Comparative Genomics; Phylogenetic Tree; Incomplete Lineage Sorting 汤鑫 PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank