2026-10-12T16:44:04Z https://www.scidb.cn/oai

10.57760/sciencedb.30842 2026-03-20T10:12:04Z

2026-03-20 Mitochondrial DNA sequencing doi:10.57760/sciencedb.30842 en To precisely quantify mitochondrial transfer efficiency, we employed a donor-recipient cell pair with distinct, naturally occurring mitochondrial DNA (mtDNA) single nucleotide polymorphisms (SNPs). HeLa cells were employed as recipient cells for mitochondrial transplantation. U2OS cells, serving as mitochondrial donors, possess a defined and homogeneous mtDNA haplogroup. Deep sequencing of the mitochondrial genome was performed, and donor-derived mtDNA was unequivocally distinguished from recipient mtDNA by tracking donor-specific SNP alleles. To investigate the potential for functional rescue, human fibroblast derived from patients harboring a high level m.3243A>G mutation and GMO4516 cell harboring a pathogenic, approximately 7.3 kb deletion in mitochondrial genome were treated with mitochondrial capsules generated from wild-type human skin fibroblast GZF2. mitochondria; mitochondrial DNA; mitochondrial DNA mutation Shiwei Du Qi Long Yanshuang Zhou Jiangqin Fu Hao Wu Liang Yang Yaohang Xie Yingzhe Ding Maolei Zhang Jingyi Guo Mengfei Wang Jiajun Lin Mingli Hu Jian Zhang Deyang Yao Wei Li Feixiang Bao Ge Xiang Yi Wu Yile Huang Haozhao Liang Rui Wang Heying Li Baodan Chen Chong Li Junwei Wang Jiwei Zhang Dajiang Qin Jianwei Sun Yun Zhu Fei Sun Wuming Wang Gang Lu Wai-Yee Chan Hui Zhao Chenli Liu Xingguo Liu PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank