2026-10-14T06:30:14Z https://www.scidb.cn/oai

10.57760/sciencedb.31047 2026-03-19T09:54:26Z

2026-03-19 Defining the Impact of Getah Virus Envelope Protein Glycosylation Site Mutations on Viral Replication, Host Adaptation, Virulence, and Immune Evasion doi:10.57760/sciencedb.31047 en Getah virus (GETV) is an emerging mosquito-borne alphavirus that causes significant animal outbreaks. Epidemic strains possess a unique N-glycosylation site (E2-N262) absent in historical lineages. The functional mechanisms of this site and conserved glycosylation structures in balancing viral replication, transmission, virulence, and immune evasion remained unclear. This study systematically investigated the impact of site-specific N-glycosylation on GETV fitness by characterizing seven glycosylation-deficient mutants. The findings reveal that these glycans are not merely structural modifications but critical determinants fine-tuning viral replication, host adaptation, virulence, and immune evasion, with evolutionary pressure favoring their retention to optimize epidemic fitness. Getah virus; N-linked glycosylation; viral replication; host adaptation; virulence; immune evasion; evolutionary selection. Zuzhang Wei Tongwei Ren PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank