2026-10-14T03:11:18Z https://www.scidb.cn/oai

10.57760/sciencedb.32336 2025-12-02T14:25:26Z

2025-12-02 Raw data from transcriptome sequencing of A549/DDP cells doi:10.57760/sciencedb.32336 en This study employed the A549/DDP human lung adenocarcinoma cisplatin-resistant cell line, which was divided into two experimental groups according to the study design: (1) blank control group (Control); (2) β-Elemene treatment group (B). Each group included three biological replicates.The experimental procedure was as follows: A549/DDP cells were expanded and seeded into 24-well plates. Transfection complexes were prepared using Opti-MEM (500 μL), Lipofectamine 2000 (20 μL), and either LINC00511 siRNA (50 μL) or an overexpression plasmid (15 μg). Cells were transfected after overnight incubation at 37 ℃ in a 5% CO₂ atmosphere. Twenty-four hours post-transfection, cells received designated treatments according to their assigned groups: final concentrations of DDP at 24 μM and β-Elemene at 40 μg/mL. Following an additional 24-hour incubation under standard culture conditions (37 ℃, 5% CO₂), cells were harvested, washed with PBS, lysed thoroughly in TRIzol reagent, snap-frozen in liquid nitrogen, and submitted to Wuhan Lingsi Biotechnology Co., Ltd. for transcriptome sequencing. Sequencing was performed on the DNBSEQ-T7RS High-throughput Sequencing System (PE150) using chemistry version V3.0. A549/DDP cell; β-Elemene; LINC00511; cisplatin resistance Deng Xiaoli PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank