2026-10-14T15:22:21Z https://www.scidb.cn/oai

10.57760/sciencedb.32381 2026-04-27T09:22:59Z

2026-04-27 Porcine reproductive and respiratory syndrome virus type 2 hijacks the host cap-dependent translation system to prioritize viral replication doi:10.57760/sciencedb.32381 en We identified host proteins interacting with PRRSV-2 N protein through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS analysis. HA-tagged N protein was expressed in HEK293T cells, lysed with NP-40 buffer, and immunoprecipitated using anti-HA magnetic beads (empty vector controls were included). The purified complexes were co-incubated with porcine alveolar macrophages (PAMs) lysates (Fig. 1A). Following this, LC-MS/MS analysis was performed on complexes pulled down by anti-HA magnetic beads binding N protein from lysates of PAM cells, alongside control pull-downs (Fig. 1A). PRRSV-2; N protein; co-immunoprecipitation Yani Sun Hui Niu PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank