2026-10-12T03:12:21Z https://www.scidb.cn/oai

10.57760/sciencedb.33230 2026-04-11T16:47:32Z

2026-04-11 CUT&Tag-seq data of ZmHsfA5-overexpressing maize plants for article "Heat shock transcription factor ZmHsfA5 coordinates with ZmHsp9 to enhance thermotolerance in maize" doi:10.57760/sciencedb.33230 en Nuclear extracts were isolated from leaves of ZmHsfA5-myc OE lines at the three-leaf stage. samples were collected at 45 °C for 0 h and 45 °C for 3 h, respectively.  Each biological replicate comprised 1.0 g leaf tissue (n = 2 replicates per group). Protein-DNA complexes were captured using the a CUT&Tag® assay kit (TD901-01, Vazyme). The experimental group employed an anti-myc antibody (ab32072, Abcam, UK), while whereas the control group used an anti-IgG antibody. Sequencing libraries were constructed following manufacturer's instructions and sequenced with 150-bp paired-end reads on an Illumina NovaSeq 6000 platform (GENEWIZ, Suzhou, Jiangsu, China).  ZmHsfA5; Heat stress; CUT&Tag-seq Qin QianQian Jiang Haiyang RESTRICTED dataset http://www.doi.org/10.1016/j.cj.2026.03.018 Science Data Bank