2026-10-14T21:54:02Z https://www.scidb.cn/oai

10.57760/sciencedb.34920 2026-01-13T10:52:12Z

2026-01-13 Identification of a novel antigenic epitope in envelope protein of avian reticuloendotheliosis virus doi:10.57760/sciencedb.34920 en Reticuloendotheliosis is a devastating poultry disease causing tumors and immunosuppression in infected birds. Caused by reticuloendotheliosis virus, it carries a surface glycoprotein (gp90) that mediates viral infection, assembly, release and induces protective antibodies, yet the specific gp90 regions triggering immune responses remain poorly understood. In this study, we expressed gp90 of a prevalent REV strain via a prokaryotic system and established a hybridoma cell line stably secreting gp90-specific monoclonal antibodies through cell fusion and flow cytometric screening. Most notably, we identified a novel linear B-cell epitope, 195REESVRERL203, on gp90 for the first time. Localized to the outer mid-domain of gp90 and conserved across REV strains, this epitope defines a key immunogenic structure, offering insights for targeted diagnostics and control strategies against this economically impactful disease. Avian reticuloendotheliosis virus; gp90; protein expression; Monoclonal antibody; antigenic epitope Jingzhe Han Mengmeng Huang Guodong Wang Yulong Zhang Runhang Liu Hangbo Yu Ziwen Wu Erjing Ke Dan Ling Suyan Wang Yuntong Chen Yongzhen Liu Yanping Zhang Hongyu Cui Yulu Duan Liuan Li Yulong Gao Xiaole Qi PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank