2026-10-14T13:02:28Z https://www.scidb.cn/oai

10.57760/sciencedb.35184 2026-07-21T16:16:42Z

2026-07-21 A single-cell time-series atlas of endothelial cell embryonic development doi:10.57760/sciencedb.35184 en Endothelial cells (ECs) are essential components of the vertebrate circulatory system; however, a comprehensive atlas characterizing how ECs acquire organ-specific transcriptomic heterogeneity has not been established. Here, we generated a time-series endothelial resource covering the entirety of mouse embryonic development, including 26 time points and 8 organs. Time-series multiorgan comparison revealed emergence timing and lineage trajectory of organotypic ECs together with organ-specific genes and pathways. Using these resources, we found that most ECs showed distinguishable organ specificity before late-gestation. The organotypic EC-enriched genes were associated with vascular function in the organs. Human and mouse pulmonary ECs underwent an evolutionarily conserved transcriptional transition. Endothelial-specific knockout of Casz1, a pulmonary EC-enriched transcription factor, resulted in impaired vascular growth, disturbed pulmonary endothelial organotypic differentiation, and deficient epithelial-ECs crosstalk. Our work provides a powerful endothelial resource that reveals fundamental principles of organ-specific ECs differentiation and uncovers previously unknown molecular mechanisms governing lung-specific vascular development. Endothelial cells; embryonic development; single cell atlas; angiogenesis; vascular development; organ specific differentiation; Casz1; lung development Li-hui Lin Jing Zhong Fu-qing Jiang Yu-xiang Wang Lan-yue_Ma Jia-xin Yang Yu-yan Li Rong-Rong Gao Huijian Feng Baomei Cai Ziyu Feng Xin Zhou Ya-hai Shu Pan Chen Xue Wu Chenlin Cai Qiang Wang Guangming Wu Shangtao Cao* Duanqing Pei* Yang Liu* Guangdun Peng* Jiekai Chen* Qi Chen* PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.1016/j.cell.2026.01.002 Science Data Bank