2026-10-14T13:31:47Z https://www.scidb.cn/oai

10.57760/sciencedb.35767 2026-05-08T14:42:28Z

2026-05-08 circRNA_013145–miR-185-5p–RhoA Axis: A Novel Mechanism in the Pathophysiology of Diabetes-induced Erectile Dysfunction doi:10.57760/sciencedb.35767 en Circular RNAs significantly impact the pathophysiology of various disorders, but their role in diabetes-induced erectile dysfunction remains unclear. We examined circular RNA expression profiles in diabetes-induced erectile dysfunction rats, identifying 641 upregulated and 647 downregulated circular RNAs. Quantitative real-time polymerase chain reaction revealed elevated circRNA_013145 levels in rat penile tissues and in high glucose-induced corpus cavernosum smooth muscle cells and human umbilical vein endothelial cells. Bioinformatics analyses identified the circRNA_013145-miRNA-mRNA regulatory network and its link to the cGMP-PKG and cAMP pathways. Interference with circRNA_013145 in high glucose-induced cells reduced inflammation, oxidative stress, phenotypic transformation, and apoptosis. Further assays confirmed that circRNA_013145 regulates RhoA by sponging miR-185-5p. Silencing circRNA_013145 improved diabetes-induced erectile dysfunction symptoms in rats, suggesting it as a potential therapeutic target.   Diabetes-Induced Erectile Dysfunction; circRNA_013145; CircRNA 黄文涵 Jianxiong Ma EMBARGO https://opendatacommons.org/licenses/by/1-0/ dataset Science Data Bank