2026-10-12T05:28:13Z https://www.scidb.cn/oai

10.57760/sciencedb.36081 2026-07-16T09:48:09Z

2026-07-16 Efficacy of docetaxel against Toxoplasma gondii in vitro doi:10.57760/sciencedb.36081 en Fresh RH tachyzoites (approximately 2×107 parasites) were incubated with docetaxel (5 nM) or 0.1% DMSO (vehicle) for 12 h at 4 ℃. Total RNA was extracted using TRIzol (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol. RNA quality was determined using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA-Library preparation and sequencing were conducted by Novogene (Beijing, China). Sequencing was performed using the Illumina platform (Illumina, San Diego, CA, USA). Clean reads were aligned to the T. gondii reference genome (GenBank accession number: PRJNA28893) using HISAT2 software. We used the DESeq to perform differentially expressed gene (DEG) analysis (P-value < 0.05). GO enrichment analysis of differentially expressed genes was implemented by the cluster Profiler R package, in which gene length bias was corrected (Yu et al., 2012). GO terms with corrected P-value < 0.05 were considered significantly enriched by differential expressed genes. We used cluster Profiler R package to test the statistical enrichment of differential expression genes in KEGG pathways. Toxoplasma gondii; docetaxel; GO enrichment; KEGG pathways Chun-Xue Zhou PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank