2026-10-14T19:45:54Z https://www.scidb.cn/oai

10.57760/sciencedb.36277 2026-07-13T13:54:23Z

2026-07-13 Genome-wide Identification of Host miRNAs Interacting with RIG-I via RIP-seq Using RIG-I as Bait doi:10.57760/sciencedb.36277 en First, HEK-293T cells were transfected with a Flag-tagged RIG-I overexpression plasmid. At 24 h post-transfection, cells were harvested and lysed, followed by RNA immunoprecipitation (RIP) assay using an anti-Flag antibody. After the specific pull-down of Flag-tagged RIG-I protein complexes by the anti-Flag antibody was verified via Western blotting (WB), total RNA was extracted from the RIP-enriched complexes and subjected to next-generation sequencing (NGS), to identify the types and relative abundance of host miRNAs bound to Flag-RIG-I. RIG-I; RIP-seq; miRNA Shiyun Chen Jiasong Xiong Lianzhong Zhao Xian Lin PUBLIC https://creativecommons.org/licenses/by-nc-sa/4.0/ dataset Science Data Bank