2026-10-12T22:25:52Z https://www.scidb.cn/oai

10.57760/sciencedb.36460 2026-05-14T18:08:01Z

2026-05-14 Optimization of strain culture medium, environmental factors, and application doi:10.57760/sciencedb.36460 en 1.5.1 Effect of CaCO3 on the denitrification performance of N. europaea W4 Set up two treatment groups without CaCO3 and 5 g/L CaCO3, with an initial ammonia nitrogen concentration of 100 mg/L. By measuring the changes in ammonia nitrogen and nitrite nitrogen, analyze the mechanism of the influence of CaCO3 on the ammonia oxidation performance of W4. 1.5.2 Effects of Fe ² ⁺ and Fe ³ ⁺ on the Denitrification Performance of N. europaea W4 Set the concentrations of Fe ² ⁺ and Fe ³ ⁺ to 0.05 g/L. Comparing the effects of different valence states of iron ions on the ammonia oxidation performance of W4 1.5.3 Effect of Temperature on the Denitrification Performance of N. europaea W4 Five temperature gradients of 10, 15, 20, 30, and 40 ℃ were set for the experiment. The inoculated conical flasks were placed in constant temperature shaking tables at corresponding temperatures and 150 r/min for cultivation. The initial concentration of ammonia nitrogen in the experiment was 100 mg/L. 1.5.4 Effect of Initial Ammonia Nitrogen Concentration on the Denitrification Performance of N. europaea W4 Determine the changes in ammonia nitrogen and nitrite nitrogen at initial concentrations of 100, 500, 1000, 1750, and 2500 mg/L. Analyze the effect of different ammonia nitrogen concentrations on the ammonia oxidation performance of strain W4. 1.6 Trial of N. europaea W4 in Aerobic Tank Wastewater Take the aerobic tank wastewater from Yichang garbage transfer station (with a daily processing capacity of 100 tons) as the treatment object. The sampling point is located in the middle of the aerobic tank (at a water depth of 1.5 meters). A sterile sampler was used to collect 25 L of mixed water samples in September 2023. After filtration through a 0.45 μ m filter membrane, the water quality indicators are shown in Table 1.1: Collect 100ml of logarithmic phase W4 culture medium and centrifuge at 3000g to prepare a suspension of bacterial cells. Resuspend the suspension and inoculate it into a 250mL conical flask containing 100mL of leachate. Incubate at 30 ℃, 50r/min, and avoid light. Set up a control group without vaccination, with 3 replicates, and measure ammonia nitrogen and nitrite nitrogen every 12 hours.  N. europaea W4; denitrification; ammonia oxidation song yi lin PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank