2026-10-12T04:44:09Z https://www.scidb.cn/oai

10.57760/sciencedb.37434 2026-05-25T18:04:05Z

2026-05-25 RNA-seq data doi:10.57760/sciencedb.37434 en This dataset comprises bulk RNA-sequencing profiles derived from colonic tumor tissues of mice, with three biological replicates per experimental group. Total RNA was isolated using TRIzol reagent, and only high-quality samples (RNA Integrity Number ≥ 6.5) assessed via NanoDrop and Agilent Bioanalyzer were processed. Messenger RNA was enriched using poly-T magnetic beads, fragmented, and converted into cDNA libraries featuring Illumina-compatible adapters, which were sequenced on a NovaSeq 6000 platform to generate 150 bp paired-end reads. The raw sequencing data were processed using fastp for quality control, aligned to the reference genome with HISAT2/TopHat2, and assembled using StringTie. Differential expression analysis was performed using edgeR or DESeq2, identifying genes with FDR < 0.05 and |log2FC| ≥ 1 as differentially expressed. Functional annotation was conducted through GO and KEGG pathway enrichment analyses using Goatools and KOBAS, respectively. RNA-seq; Colon cancer; Differentially expressed genes Qingkun RESTRICTED dataset Science Data Bank