2026-10-14T14:45:29Z https://www.scidb.cn/oai

10.57760/sciencedb.37441 2026-05-25T18:04:03Z

2026-05-25 16S rRNA doi:10.57760/sciencedb.37441 en This dataset comprises 16S rRNA gene sequencing data (V3–V4 region, primers 341F/806R) generated from mouse fecal samples using the E.Z.N.A.® Stool DNA Kit, with sequencing performed on the Illumina NovaSeq 6000 platform (PE250 mode). It includes samples from AOM/DSS and APCmin/+ intervention cohorts, as well as n=5 mice per group from antibiotic depletion (ABX) and fecal microbiota transplantation (FMT) experiments. Raw reads were processed via QIIME 2 and DADA2 for denoising and amplicon sequence variant (ASV) inference, with taxonomic assignment using the SILVA database. Downstream analyses include alpha diversity (Shannon, Chao1), beta diversity (Bray-Curtis PCoA), and differential abundance testing via LEfSe (LDA > 2.0). AOM/DSS intervention cohorts; APCmin/+ intervention cohorts; ABX-depletion/FMT experiments Qingkun RESTRICTED dataset Science Data Bank