2026-10-14T11:21:30Z https://www.scidb.cn/oai

10.57760/sciencedb.37529 2026-08-17T11:21:11Z

2026-08-17 Viral metagenomic of the diseased cattle doi:10.57760/sciencedb.37529 en Between August 2023 and August 2024, a total of 1,518 animal samples were collected across 19 provinces and autonomous regions, as well as 3 municipalities in China. Sample types comprised nasal swabs (n=393), anal swabs (n=642), vaginal swabs (n=42), fecal samples (n=124), serum (n=6), and organ tissues (lung, liver, spleen, kidney, intestinal tract, heart, and uterine contents) (n=311) (Figure 1a). According to sample origin and type, 54 sequencing libraries were constructed, including 12 respiratory-associated libraries, 28 digestive tract-associated libraries, and 14 mixed libraries.For MTT, RNA was extracted using Trizol reagent(Invitrogen, Carlsbad, California, USA) and rRNA depletion was performed using Ribo-ZeroTM Magnetic Gold Kit (Epicentra Biotechnologies, Madison,, USA), RNA sequencing was performed on an Illumina NovaSeq sequencer using NEBNext Super-Directed RNA Library Prep Reagents cassette (New England Biolabs, Ipswich, USA). For MDA, DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) and amplified using the illustra GenomiPhi V2 DNA Amplification Kit (GE, Fairfield,, USA). Products were purified using the QIAquick PCR purification Kit (Qiagen, Hilden, Germany), followed by Illumina paired-end sequencing (150 bp) on an Illumina NovaSeq 6000 sequencer. Virome; Cattle; Disease Sunyue PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank