2026-10-14T23:38:22Z https://www.scidb.cn/oai

10.57760/sciencedb.37697 2026-05-25T11:32:14Z

2026-05-25 Construction of Silver Nanoparticle-based Artificial Hydrolases via Conformational Engineering and Study of Its Catalytic Mechanism doi:10.57760/sciencedb.37697 en This dataset originates from a study on the construction of a highly active silver-based artificial hydrolase (Silverzyme) based on conformational engineering strategies. Using silver nanoparticles (AgNPs) as the scaffold, the study successfully mimicked the catalytic center of α-chymotrypsin—including the catalytic triad, oxyanion hole, and substrate-binding site—through peptide design and a three-step conformational control method (induction of α-helical conformation by trifluoroethanol, stabilization of the conformation via Ag–S bonds, and removal of the inducer by freeze-drying). The dataset primarily includes the following content:Circular Dichroism (CD) data: Used to demonstrate the formation and maintenance of the α-helical conformation of the peptides on the AgNP surface, thereby validating the successful implementation of conformational engineering.Stopped-flow kinetics data: Including comparisons of catalytic activity before and after mutation of key residues, used to demonstrate the functional necessity of the catalytic triad, oxyanion hole, and substrate-binding site, as well as their synergistic effects.Catalytic activity data: The turnover number per active site measured using p-nitrophenyl acetate (p-NPA) as the substrate.Substrate scope expansion data: Activity data showing the catalytic degradation of diethyl phthalate (DEP), an environmental priority pollutant, by Silverzyme, demonstrating its ability to hydrolyze non-activated esters. artificial enzyme; enzyme-mimicking; silver nanoparticles wang yan Cao Aoneng Zhou Tongtong PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset http://www.doi.org/10.3724/j.pibb.2026.0152 Science Data Bank