2026-10-13T16:54:47Z https://www.scidb.cn/oai

10.57760/sciencedb.37761 2026-05-22T17:13:40Z

2026-05-22 Experimental data on using the gentamicin-loaded silk-based suture anchor to repair the rotator cuff of rabbits. doi:10.57760/sciencedb.37761 en Part 1. This part of the data mainly consists of the original images from the experiments, including the rabbit rotator cuff injury model, samples taken at different time points, and the biomechanical experiment process. Materials and methods. The bilateral shoulder joints of eighteen New Zealand rabbits were operated on: A sharp knife was used to make a 5 mm horizontal incision on the rotator cuff to simulate rotator cuff injury. The bone was debrided at the rotator cuff insertion point, and a drill bit with a diameter of 4 mm was used to drill and tap a hole. The GSSA was screwed into the left shoulder joint using a hollow hexagonal screw, and the damaged rotator cuff was repaired and fixed to the bone surface using a mattress suture. For the right shoulder joint (control), a 1-mm drill bit was used to drill two holes in the proximal end of the insertion point, and a No. 2 PANACRYL suture was used for direct mattress suture from bottom to top to repair the damaged rotator cuff without an anchor. Imaging evaluation, histological evaluation, and rotator cuff biomechanical testing were performed at 2, 4, and 8 weeks after surgery.In 10 New Zealand rabbits, the GSSAs were screwed into the supraspinatus tendon insertion of the bilateral humerus according to the previous steps, and the anchor sutures were placed subcutaneously. The humerus was completely removed (4 specimens) at each time point: immediately, 3 days, 2 weeks, 4 weeks and 8 weeks after surgery. The humerus and the suture were fixed at both ends of the biomechanical testing machine (CMT-4304HT, SUST, China), keeping the suture and the anchor in the same direction (Fig. 2c), and the specimen was prestretched using a tension of 2 N for 20 seconds. Pullout tests were performed at a rate of 10 mm/min until the anchors failed. The maximum tensile strength of the GSSA is the maximum tensile force during the stretching process.The humerus and rotator cuff tissue were completely removed and fixed on a biomechanical testing machine with the longitudinal axis of the humerus and the rotator cuff positioned in the same direction. Afterward, the sample was prestretched using a tension of 2 N, and the tensile test was carried out at a speed of 10 mm/min. The maximum tensile strength of the rotator cuff is the maximum tensile force that the tendon‒bone interface can withstand during the stretch test.Part 2. This part of the data mainly consists of hematological test results in vivo.Materials and methods. Eighteen New Zealan d rabbits were divided into three groups of6 rabbits in each group, numbered and weighed, and then anesthetized by the injection of xylazine hydrochloride and 3% pentobarbital. The left forelimb shoulder area was shaved and disinfected. After skin incision and muscle retraction, the supraspinatus tendon insertion was exposed. A 4-mm-diameter drill bit was used to drill a hole at the insertion point, and the thread was manually tapped. The gentamicin-loaded silk-based anchor and the pure silk-based anchor were screwed into place in groups A and B, respectively, and in group C, the surgical procedure was performed without implantation of the anchor. The wound was sutured and bandaged, and penicillin was injected intramuscularly after surgery. The activities of the rabbits were not restricted. Blood samples were collected from the ear vein of the rabbits before surgery and at 1 day, 3 days, and 7 days after surgery for blood chemical analysis (BCA), C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and serum creatinine (SCR) levels. Serum potassium (K+) and serum sodium (NA+) levels were also assessed.Part 3. This part of the data mainly consists of the maximum pullout strength of the gentamicin-loaded silk-based suture anchorin in vivo.The materials and methods have been described in the part 1.Part 4. This part of the data mainly consists of the maximum tensile strength of the rotator cuff in vivo.The materials and methods have been described in the part 1.Part 5. This part of the data mainly consists of the original imaging images and measurement data.Materials and methods. Micro-computed tomography (micro-CT, SkyScan 1276, Bruker, Germany) and magnetic resonance imaging (MRI, Optima MR360 1.5T, GE, USA) were used to scan the bilateral shoulder joints to evaluate the morphology and position of the anchor in the humerus and the inflammation of the surrounding tissues. The diameter and length of the anchor were measured by micro-CT at 3 days, 2 weeks, 4 weeks, 8 weeks, 12 weeks and 24 weeks after surgery. The expansion and decrease rates were calculated according to the above measurement results.Part 6. This part of the data mainly consists of the pathological section and organization scores.Materials and methods.The healing status of the rotator cuff, the appearance and location of the anchor, and the presence of local infection were evaluated. Afterward, the shoulder joint was completely removed and fixed with 4% paraformaldehyde, which was completely decalcified using ethylenediaminetetraacetic acid (EDTA) decalcification solution, embedded in paraffin, sectioned, and stained using hematoxylin and eosin (HE) and Masson’s trichrome staining for observation under a light microscope. Local tissue and cellular responses in HE-stained sections were assessed according to the modified International Organization for Standardization (ISO) 10993-6, specifically, the inflammatory reaction (infiltration of neutrophils, lymphocytes, and plasma cells), phagocytic reaction (macrophages and giant cells), and tissue reaction (extent of neovascularization, fibrosis, and fatty infiltration). the gentamicin-loaded silk-based suture anchor; biomechanical research; expansion and decrease; biocompatibility study Chenglong Shi Ruihui Wu Xiushi Li Guan Zheng Feng Lin Xingqiu Huang Shuai Huang Donghai Deng Yunli Mei Hong Xia EMBARGO https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank