2026-10-15T03:43:50Z https://www.scidb.cn/oai

10.57760/sciencedb.38022 2026-05-25T16:03:24Z

2026-05-25 Astragalus Polysaccharide Ameliorates Myocardial Ischemia by Regulating BCL-2 Signaling-Mediated Mitochondri-al Function: A Combined Bioinformatics and Experimental Study doi:10.57760/sciencedb.38022 en Acute myocardial infarction (AMI) is a major cause of cardiovascular mortality, driven by mitochondrial dysfunction, apoptosis, and inflammation. This study aimed to identify AMI biomarkers and explore the cardioprotective mechanisms of Astragalus polysaccharides (APS). Bioinformatics analysis of GEO datasets, weighted gene co‑expression network analysis, and machine learning identified four mitochondrial‑related biomarkers, with ACSL1 and BCL2A1 closely correlated with the NF‑κB pathway. A rat AMI model was established via coronary ligation and treated with APS for 21 days. APS improved cardiac function, reduced infarct size, ameliorated mitochondrial ultrastructure damage, and promoted angiogenesis. Mechanistically, APS upregulated BCL‑2, downregulated pro‑apoptotic proteins, and inhibited NF‑κB p65 phosphorylation. ACSL1 and BCL2A1 represent potential AMI biomarkers. APS protects against myocardial injury by modulating apoptosis, inflammation, and angiogenesis, supporting its therapeutic potential for AMI. Myocardial infarction; Astragalus polysaccharides; BCL-2 signaling pathway; Mito-chondrial function; Bioinformatics Weili Shi Shanshan Liu Chaoqun Lu Peishuo Yan Xinzhou Wang Yawei Zhang Nan Qin Qiyang Wang Pengfei Lu PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank