2026-10-14T13:57:33Z https://www.scidb.cn/oai

10.57760/sciencedb.38467 2026-06-01T14:58:30Z

2026-06-01 Transcriptome raw data from control, bollworm-infested, and wounded tobacco plants doi:10.57760/sciencedb.38467 en NC89 tobacco plants were grown in a growth chamber with a 14 h light/10 h darkness photoperiod and temperature controlled at 25 ± 3 °C. The eggs of cotton bollworm (Helicoverpa armigera) were purchased from Keyun Industrial Co., Ltd. in Jiyuan, Henan. The larvae were reared under dark conditions in an insect breeding room (25 ± 3 °C) and 2nd to 3rd instar larvae were used for biological assays. Twenty-day-old tobacco plants were divided into three groups: check (CK), 5th instar Helicoverpa armigera larvae-infested plants (infested), and mechanically damaged plants (wounded). Total RNA was extracted from the check (CK_1, CK_2, CK_3), infested (Infested_1, Infested_2, Infested_3), and wounded (Wound_1, Wound_2, Wound_3) plants using the TRIzol™ reagent (Invitrogen) according to the manufacturer’s instructions. Polyadenylated mRNA was purified using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich，USA). Illumina cDNA libraries were prepared with the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA) following the manufacturer’s protocol, and sequencing was performed on an Illumina HiSeq 2000 platform. bollworm; RNA-SEQ; WOUND LI PING PUBLIC https://creativecommons.org/licenses/by-nc-sa/4.0/ dataset http://www.doi.org/10.1016/j.cj.2026.04.010 Science Data Bank