2026-10-12T06:36:13Z https://www.scidb.cn/oai

10.57760/sciencedb.38546 2026-06-16T11:28:13Z

2026-06-16 Functional heterogeneity of mouse fetal liver hematopoietic stem cells revealed by clonal lineage tracing and single-cell transcriptomics doi:10.57760/sciencedb.38546 en To simultaneously capture mRNA and lineage information from individual HSC clones, we labeled E14.5 fetal liver ESLAM‑HSCs (CD45⁺CD201⁺CD48⁻CD150⁺) with a lentiviral LARRY barcode library and transplanted them into lethally irradiated primary recipient mice. After confirming long‑term multilineage reconstitution, we sorted GFP⁺ hematopoietic stem and progenitor cells from the bone marrow of primary recipients. Half of the sorted cells were processed for single‑cell RNA sequencing, while the remaining HSCs were injected into secondary recipients to assess self‑renewal potential. Following long‑term engraftment in secondary recipients, GFP⁺ HSPCs were similarly isolated. For both primary and secondary transplantation cohorts, a defined mixture of LT‑HSCs and other progenitor populations (MPPs, MyPs, MkPs, CLPs) was prepared to reconstitute the full hematopoietic stem and progenitor compartment. In total, samples from five primary and five secondary recipients were used to generate the dataset. The mixed cell suspensions were processed for single‑cell RNA sequencing using the 10× Genomics 3′ scRNA‑seq kit according to the manufacturer’s protocol, and libraries were sequenced on an Illumina NovaSeq platform with paired‑end 150 bp reads. Fetal liver; Hematopoietic stem cell; Clonal barcode tracing; Single-cell RNA sequencing; Heterogeneity Man Zhang Yanli Ni Di Liu Dong Xiang Gaoke Liu Jie Zhou Yu Lan Bing Liu Zongcheng Li EMBARGO https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank