2026-10-14T12:24:49Z https://www.scidb.cn/oai

10.57760/sciencedb.38857 2026-06-08T11:35:56Z

2026-06-08 New records and an updated checklist of the genus Myotis (Chiroptera, Vespertilionidae) in Xizang, China doi:10.57760/sciencedb.38857 en Morphological data collectionFollowing the Chiroptera Morphometric Standard (Yang et al., 2007), 5 external and 13 craniodental characteristics (Table 2; Supplementary Table S2) were taken using MNT-150 digital calipers (Meinert Industrial Co., Ltd., China; accuracy 0.01 mm). External measurements included forearm length (FA); tibia length (TIB); hindfoot length including claw (HF); ear length (EL); and tail length (TL). Craniodental measurements comprised: greatest length of skull (GTL); condylocanine length (CCL); zygomatic breadth (ZB); mastoid width (MAW); braincase width (BCW); height of braincase (BCH); palatal bridge length (PBL); maxillary toothrow length (C1M3L); upper canine width (C1C1W); width across the upper molars (M3M3W); mandibular toothrow length (C1M3L); mandible length (ML); and mandibular height (MH). Missing values were absent in our morphometric dataset.Genetic data (cyt b) acquisitionTotal genomic DNA was extracted from approximately 20 mg of muscle tissue using a commercial kit (DNU333-03, Maibao Biotechnology Co., Ltd., China). The mitochondrial cytochrome b (cyt b) gene was amplified by PCR using primers from Irwin et al. (1991) and Ruedi et al. (2013). Each 30 µL reaction contained: 13 µL of 2×Accurate Taq Premix (AG11009, Accurate Biotechnology Co., Ltd., China), 0.5 µL of each forward and reverse primer (10 µmol/L), 14 µL of RNase-free water, and 2 µL of DNA template (~20 ng/µL). Thermal cycling profile was: initial denaturation at 94 °C for 5 min; 37 cycles of 94 °C for 45 s, 46 °C for 30 s, and 72 °C for 60 s; and a final extension at 72 °C for 5 min. Amplification products were verified by agarose gel electrophoresis and submitted to Sangon Biotech (Shanghai) for bidirectional Sanger sequencing. The 18 newly generated cyt b sequences from this study.Echolocation call recordingEcholocation calls of Myotis bats were recorded in flight state using an ultrasound detector (M500-384, Pettersson Elektronik AB, Sweden). Recordings were conducted in a quiet room (approximately 3 m×3 m×3 m). Acoustic data were analyzed using BatSound v.4.0 (sampling frequency: 300 kHz, Fast Fourier Transform (FFT) size: 1024 points). For each species, more than 10 high-quality pulses were selected to measure three key parameters: peak frequency, pulse duration, and inter-pulse interval. Given that the Myotis calls typically consist of broadband frequency-modulated (FM) signals with multiple harmonics, where the first harmonic is the strongest and other harmonics (2nd–4th) decrease successively, only the first harmonic was analyzed in this study. Xizang (China); New records; Myotis; Species diversity; Taxonomic revision Kun-Hao Chen Yang Guo Yong-Xin Miao Lin-Ying Xue Yi Wu Yang Yue Li-Biao Zhang Wen-Hua Yu Xiao-Yun Wang PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank